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Flow Cytometry and FACS Core Facility

  • Managing PI: Martin Hudson

    Sony Biosciences SH800 Cell Sorter. The SH800 cell sorter is equipped with four lasers (405nm, 488nm, 561nm and 638nm) and six fluorescence detectors in addition to forward and backscatter detectors. This laser setup permits maximum flexibility in experimental design and allows easy discrimination between live cell labels such as GFP and RFP or Cherry. Disposable sorting chips featuring microfluidic technology are used to combine the sheath and sample lines. Fluidics, laser alignment, and sort stream calibration are all fully automated. The system can be run in analytical and preparative sort modes. Multiple sample tubes can be accommodated (0.5ml microfuge tube up to a 35ml centrifuge tube) and a wide variety of collection systems are available for cell sorting runs, including a 96 well-format collector that can be used in single cell sort mode.  National Science Foundation (NSF) logo The entire instrument is housed in a custom-built Baker BSL class IIA biosafety cabinet, allowing the safe sorting of potentially hazardous samples. The system is controlled by proprietary Sony software and is very easy to set up and use. Please contact Martin Hudson for training and scheduling. This instrument was purchased via a NSF MRI grant.

  • Managing PI: Martin Hudson

    BD Acurri C6 Flow Cytometer and software The Accuri C6 flow cytometer is equipped with 488nm and 647nm lasers, and four fluorescence detectors that can arranged in different modes via the use of interchangeable filters. Particle size and granularity are measured using forward and side-scatter detectors. The system is powered by BD software, and data can be exported in multiple file formats, including FCS-3 standard. The instrument is very easy to set up and run, and utilizes distilled water as the sheath fluid. Please contact Martin Hudson for training and scheduling. Georgia Research Alliance logo This instrument was purchased via a donation from the Georgia Research Alliance.

  • FlowJo logo An iMac equipped with FlowJo is available for off-line FACS and flow cytometry data analysis. This can be used for post-acquisition signal compensation, data analysis, and figure preparation for publication. Please contact Martin Hudson for more information.
  • Photo of Flow cytometry analysis of eGFP and mCherry transfected 293T cells. Data captured on the Sony SH800 cell sorter. Photo courtesy of Martin Hudson Flow cytometry analysis of eGFP and mCherry transfected 293T cells. Data captured on the Sony SH800 cell sorter. Photo courtesy of Martin Hudson.

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